Phylogenetic Tree Builder

iNaturalist Observation URL or ID or username or project

Enter an observation ID, observation URL, username, user URL, project name, or project URL.

Mycomap BLAST Results URL

Paste a Mycomap BLAST result URL to import sequences.

NCBI Results Local Results Remove Duplicates

Enter Single Sequence or Accession

Find related sequences on NCBI GenBank.

Min Identity (%)

Max Results

Click to upload or drag and drop

FASTA files (.fa, .fasta, .fna, .txt)

Job Name / Notes

Paste FASTA Sequences or GenBank Accessions

Enter FASTA sequences or one or more GenBank accessions.

No sequences added yet.

Paste FASTA sequences and click "Add / Remove Sequences"

Pipeline: MAFFT → FastTree · GTR+CAT · 1000 BS · midpoint root

Import MycoMap results to enable filtering by query coverage, subject coverage, and identity.

Advanced

Currently: MAFFT + FastTree 2.2.0, midpoint-rooted, 1000 bootstrap.

Alignment Method

Choose the algorithm for multiple sequence alignment.

Trimming Method

Remove poorly aligned regions to improve signal.

Method

Substitution Model

E.g., GTR+G, JC69, HKY.